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hemQ [2017-09-04 16:36:41]
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hemQ [2017-09-04 16:36:41]

coproheme decarboxylase
Locus
BSU37670
Isoelectric point
4.97
Molecular weight
29.35 kDa
Protein length
254 aa Sequence Blast
Gene length
762 bp Sequence Blast
Function
biosynthesis of heme
Product
coproheme decarboxylase
Essential
no
E.C.
1.11.1.-
Synonyms
ipa-87r,ywfI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis of heme/ siroheme
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    3,866,596 → 3,867,360

    Phenotypes of a mutant

  • defect in heme biosynthesis

    • The protein

    Catalyzed reaction/ biological activity

  • coproheme --> protoheme + 2 CO2 + 4 H+ PubMed

    • Protein family

  • UPF0447 family (according to Swiss-Prot)

    • Modification

  • phosphorylation on Ser-41 PubMed

    • Cofactors

  • heme

    • Structure

  • 1T0T (Geobacillus stearothermophilus)

    • Expression and Regulation

    Operons

    Genes
    hemQ
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-A516 (ywfI::erm), available at the NBRP B. subtilis, Japan

    • Expression vector

  • pGP2643: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
  • pGP2644: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
  • pGP2637: N-terminal Strep-tag, in pGP380, for SPINE, available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

    • References

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